A simple and rapid purification method is described which exploits the heat stability of human tau (tau) protein to prepare truncated forms of this protein derived from bacteria. Bacterial cells expressing tau fragments were pelleted, resuspended in phosphate buffered saline and boiled for 5 min. After centrifugation the supernatant containing thermostable tau was filtered (0.45 microns) and used for immunoanalysis with monoclonal antibodies. The purified tau fragments exhibited identical antigenic properties as fragments isolated by a conventional procedure, based on ion exchange chromatography on phosphocellulose. In contrast to the conventional approach, our method is less complicated, cheaper and significantly reduces the time required for isolation of the recombinant tau fragments.

Quick purification of recombinant human truncated tau proteins for immunoanalysis

CATTANEO, ANTONINO;
1995

Abstract

A simple and rapid purification method is described which exploits the heat stability of human tau (tau) protein to prepare truncated forms of this protein derived from bacteria. Bacterial cells expressing tau fragments were pelleted, resuspended in phosphate buffered saline and boiled for 5 min. After centrifugation the supernatant containing thermostable tau was filtered (0.45 microns) and used for immunoanalysis with monoclonal antibodies. The purified tau fragments exhibited identical antigenic properties as fragments isolated by a conventional procedure, based on ion exchange chromatography on phosphocellulose. In contrast to the conventional approach, our method is less complicated, cheaper and significantly reduces the time required for isolation of the recombinant tau fragments.
1995
File in questo prodotto:
File Dimensione Formato  
Kontsekova Cattaneo J Immunol Methods 1995.pdf

Accesso chiuso

Tipologia: Altro materiale allegato
Licenza: Non pubblico
Dimensione 229.68 kB
Formato Adobe PDF
229.68 kB Adobe PDF   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11384/1152
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact