Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors

Marchetti, Laura; Bonsignore, Fulvio; Amodeo, Rosy; Schirripa Spagnolo, Chiara; Moscardini, Aldo; Gobbo, Francesco; Cattaneo, Antonino; Beltram, Fabio; Luin, Stefano

doi:10.1117/12.2578193

We exploited, optimized, and developed various microscopy techniques for analyzing living matter at cellular and molecular levels. In particular, we developed a toolbox for single particle tracking (SPT) of membrane receptors and their ligands, suitable also for relatively fast single-pass membrane receptors; this is based on chemical tagging of recombinant proteins, TIRF microscopy, and automatized analysis of single particle trajectories. We are now extending it to simultaneous visualization and analysis of two moieties. The superresolved localization of this technique allowed analyzing functions, interactions and stoichiometry of (pro)neurotrophin receptors p75NTR and TrkA and of their ligands in living cells, with particular attention on some of their existing or used mutants. The analysis also after treatments with ligands or drugs unraveled their mode of action in the first steps of sundry signaling pathways.

Titolo:	Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors
Autori:	MARCHETTI, LAURA BONSIGNORE, FULVIO Amodeo, Rosy Schirripa Spagnolo, Chiara MOSCARDINI, ALDO GOBBO, FRANCESCO CATTANEO, ANTONINO BELTRAM, Fabio LUIN, Stefano
Titolo del libro:	PROCEEDINGS VOLUME 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV
Data di pubblicazione:	2021
Nome del convegno:	Single Molecule Spectroscopy and Superresolution Imaging XIV
Settore Scientifico Disciplinare:	Settore FIS/03 - Fisica della Materia Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) Settore BIO/09 - Fisiologia Settore CHIM/06 - Chimica Organica
Digital Object Identifier (DOI):	http://dx.doi.org/10.1117/12.2578193
URL:	https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11650/2578193/Single-molecule-tracking-and-spectroscopy-unveils-molecular-details-in-function/10.1117/12.2578193.short
Handle:	http://hdl.handle.net/11384/101364
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