Fluorescence lifetime microscopy unveils the nanoscale organization of liposomal Doxorubicin

The present invention relates to determination of supramolecular organization in a substance including target molecules and nanocarriers at least one of which is luminescent, based on a step of collecting of lifetime decay data of at least a standard substance pure or substantially pure wherein a known organization state of the target molecules and the nanocarriers is pure or substantially pure; and a step of comparing the standard data and test data from a test substance.