Fluorescence lifetime analysis (FLA) has become essential for non-invasive, real-time analysis of a variegate realm of compounds and materials in scientific and industrial fields. Here we present a compact and versatile device designed to apply FLA to the screening of healthcare nanoformulations which contain a luminescent active principle. In the case study here: Doxil FLA enables to decode the supramolecular organization and stability of the luminescent active principle within the nanoformulation in a non-invasive, rapid (seconds), and cost-effective manner. Contrary to currently used methods (e.g. TEM, SEM, CryoEM, HPLC), this device performs the analysis in the natural solvent with no need for sample chemical manipulation or labelling. This advancement holds promise for enhancing research and quality control in pharmaceutical industries. Also, we envision in the near future application of this technology to evaluate drug entry into cells and to monitor the change of drug supramolecular organization upon contact with biological environments, such as biological fluids, cells, tissues.

Fluorescence lifetime analysis (FLA) for the screening of healthcare nanoformulations: towards a compact and versatile device

CARDARELLI, Francesco
2024

Abstract

Fluorescence lifetime analysis (FLA) has become essential for non-invasive, real-time analysis of a variegate realm of compounds and materials in scientific and industrial fields. Here we present a compact and versatile device designed to apply FLA to the screening of healthcare nanoformulations which contain a luminescent active principle. In the case study here: Doxil FLA enables to decode the supramolecular organization and stability of the luminescent active principle within the nanoformulation in a non-invasive, rapid (seconds), and cost-effective manner. Contrary to currently used methods (e.g. TEM, SEM, CryoEM, HPLC), this device performs the analysis in the natural solvent with no need for sample chemical manipulation or labelling. This advancement holds promise for enhancing research and quality control in pharmaceutical industries. Also, we envision in the near future application of this technology to evaluate drug entry into cells and to monitor the change of drug supramolecular organization upon contact with biological environments, such as biological fluids, cells, tissues.
2024
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
SPIE BiOS
usa
2024
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV
SPIE
9781510669833
doxorubicin; FLA; fluorescence lifetime; nanomedicine;
   CAPTURING THE PHYSICS OF LIFE ON 3D-TRAFFICKING SUBCELLULAR NANOSYSTEMS (CAPTUR3D)
   CAPTUR3D
   European Commission
   H2020
   866127
File in questo prodotto:
File Dimensione Formato  
2024_SPIE_Proceedings___Paper_Number_12862_27__Fluorescence_lifetime_analysis__FLA__for_the_screening_of_healthcare_nanoformulations.pdf

Accesso chiuso

Descrizione: articolo
Tipologia: Published version
Licenza: Non pubblico
Dimensione 349.13 kB
Formato Adobe PDF
349.13 kB Adobe PDF   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11384/143323
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
  • OpenAlex ND
social impact