A deep learning-based dataset of WFA-positive perineuronal nets and parvalbumin neurons localizations in the adult mouse brain

Quality-controlled predictions of deep learning models for cell counting This dataset contains high-resolution images for the visualization of perineuronal nets (PNNs) and parvalbumin-expressing (PV) cells analyzed in the paper: A Comprehensive Atlas of Perineuronal Net Distribution and Colocalization with Parvalbumin in the Adult Mouse Brain. The dataset integrates the raw data published on a previous upload on Zenodo. Cell locations were obtained using two deep-learning models for cell counting (publicly available on GitHub, details in the paper by Ciampi et al., 2022). The output of the deep-learning pipeline was filtered based on the score assigned to each cell prediction, by removing all the PNNs with a score lower than 0.4 and all the PV cells with a score lower than 0.55. Cases of artefactual cell detection were finally removed manually by visual inspection of the images. Content The dataset contains microscopy images of coronal brain slices from 7 adult mice. The objects highlighted in these images represent the final set of PNNs/PV cells that were used in all the analysis of the paper. Folder Structure and file naming conventions There are separate folders for each mouse. Each folder is named with the ID of that mouse. Within each folder, images are assigned a code specifying the channel (C1 for PNNs, C2 for PV cells).