In this study we exposed 1,2-dioleoyl-3-trimethylammonium propane (DOTAP)/DNA lipoplexes to different concentrations of human plasma (HP). Liquid chromatography-tandem mass spectrometry showed that the adsorbed protein corona is rich in vitronectin, a major ligand for Î± vÎ² 3 or Î± vÎ² 5 integrins. To investigate whether the protein corona could dictate a selective access to cells expressing vitronectin receptors, highly-metastatic MDA-MB-435S cells that express high levels of Î± vÎ² 3 and Î± vÎ² 5 integrins, were used as experimental model. Non-metastatic MCF7 cells that do not express Î± vÎ² 3 and express reduced levels of Î± vÎ² 5 integrins were used as a reference. After exposure to HP, the cellular uptake of lipoplexes in MDA-MB-435S cells was more than 2-fold larger than that of bare ones, while complexes could not enter MCF7 cells remaining largely accumulated at the plasma membrane. Collectively our data suggest that the protein corona can be used as a novel tool for cancer cell targeting.
|Titolo:||Cancer cell targeting of lipid gene vectors by protein corona|
|Titolo del libro:||Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012|
|Data di pubblicazione:||2012|
|Nome del convegno:||Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012|
|Parole Chiave:||Cationic liposome; DNA; Gene delivery; Lipoplexes; Protein corona; Ceramics and Composites; Surfaces, Coatings and Films|
|Appare nelle tipologie:||4.1 Contributo in Atti di convegno|