Surface functionalized multicomponent envelope type nanosystems for improved gene delivery

Multicomponent envelope-type lipid nanosystem (MENS) is an emerging nanoparticle technology. These nanoparticles were assembled according to a recently proposed 'programmed packaging concept' in which various devices that control intracellular trafficking are packed into a single multicomponent envelope-type nanoparticle system. The uptake ability and final intracellular fate of MENS formulations was evaluated by means of confocal laser scannig microscopy. Our results revealed that MENS show a high cellular internalization and that are not specifically targeted to metabolic degradation. These studies will contribute to rationally design novel delivery systems with superior transfection efficiency.