We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used.

Site-specific direct labeling of neurotrophins and their receptors: From biochemistry to advanced imaging applications

Gobbo, Francesco;Bonsignore, Fulvio;Amodeo, Rosy;Cattaneo, Antonino;Marchetti, Laura
2018

Abstract

We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used.
Methods in Molecular Biology
Humana Press Inc.
Astrocyte culture; Axonal transport; Biotinylation; Fluorescence labeling; Inducible lentivirus; Membrane receptor pool; Nerve growth factor; Neuron culture; Neurotrophin; TrkA tyrosine kinase; Molecular Biology; Genetics
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11384/76810
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