The ability to perform in vitro fertilization, together with sperm cryopreservation, greatly facilitates the long-term laboratory maintenance of wild-type and transgenic model organisms and helps prevent genetic drift. It is also useful in cases where reproduction may be compromised. In this protocol, we present a method for in vitro fertilization of the African Turquoise killifish Nothobranchius furzeri that is compatible with the use of fresh or cryopreserved sperm.

In Vitro Fertilization of the African Turquoise Killifish Nothobranchius furzeri

Dolfi, Luca;Ripa, Roberto;
2023

Abstract

The ability to perform in vitro fertilization, together with sperm cryopreservation, greatly facilitates the long-term laboratory maintenance of wild-type and transgenic model organisms and helps prevent genetic drift. It is also useful in cases where reproduction may be compromised. In this protocol, we present a method for in vitro fertilization of the African Turquoise killifish Nothobranchius furzeri that is compatible with the use of fresh or cryopreserved sperm.
2023
Settore BIO/09 - Fisiologia
Settore MVET-01/B - Fisiologia veterinaria
Aging; Animals; Fertilization in Vitro; Fundulidae; Laboratories; Male; Nothobranchius furzeri; Semen; in vitro fertilization; killifish; male; nonhuman; sperm; aging; animal; Fundulidae; laboratory
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11384/146366
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