A F. oxysporum f. sp. dianthi strain, transformed with genes coding for fluorescent proteins (GFP or DsRedFP) as markers, was used to study the first host/pathogen interaction on carnation roots. The transformants’ mycelium observed under fluorescent light displayed a high expression of GFP and DsRedFP as a bright green or red cytoplasmic fluorescence. The root apparatus of a partially resistant cultivar of carnation was artificially inoculated by stable transformants and local colonization of plant tissues was monitored by means of fluorescence microscopy. A GFP transformed strain of F. oxysporum f. sp. dianthi allowed to follow first colonization steps on and within host root tissues. Implication of this research in studying resistance processes in carnation is discussed.

Fusarium oxysporum f. sp. dianthi transformed with marker genes as a tool for studying resistance in Dianthus

VERGARA, Mariarosaria;
2007

Abstract

A F. oxysporum f. sp. dianthi strain, transformed with genes coding for fluorescent proteins (GFP or DsRedFP) as markers, was used to study the first host/pathogen interaction on carnation roots. The transformants’ mycelium observed under fluorescent light displayed a high expression of GFP and DsRedFP as a bright green or red cytoplasmic fluorescence. The root apparatus of a partially resistant cultivar of carnation was artificially inoculated by stable transformants and local colonization of plant tissues was monitored by means of fluorescence microscopy. A GFP transformed strain of F. oxysporum f. sp. dianthi allowed to follow first colonization steps on and within host root tissues. Implication of this research in studying resistance processes in carnation is discussed.
2007
Carnation; F. oxysporum f. sp. dianthi; fluorescent reporter proteins; GFP; DsRed; root colonization
File in questo prodotto:
File Dimensione Formato  
Vergara Acta Phytopathol Entomol Hungarica 2007.pdf

Accesso chiuso

Tipologia: Altro materiale allegato
Licenza: Non pubblico
Dimensione 544.2 kB
Formato Adobe PDF
544.2 kB Adobe PDF   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11384/3409
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
  • OpenAlex ND
social impact