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EnglishThe membrane-destabilization properties of the recently-introduced endosomolytic CM18-Tat11 hybrid peptide (KWKLFKKIGAVLKVLTTG-YGRKKRRQRRR, residues 1-7 of cecropin-A, 2-12 of melittin, and 47-57 of HIV-1 Tat protein) are investigated in CHO-K1 cells by using the whole-cell configuration of the patch-clamp technique. CM18-Tat11, CM18, and Tat11 peptides are administered to the cell membrane with a computer-controlled micro-perfusion system. CM18-Tat11 induces irreversible cell-membrane permeabilization at concentrations (≥4 µM) at which CM18 triggers transient pore formation, and Tat11 does not affect membrane integrity. We argue that the addition of the Tat11 module to CM18 is able to trigger a shift in the mechanism of membrane destabilization from "toroidal" to "carpet", promoting a detergent-like membrane disruption. Collectively, these results rationalize previous observations on CM18-Tat11 delivery properties that we believe can guide the engineering of new modular peptides tailored to specific cargo-delivery applications.
| Titolo: | Mechanistic insight into CM18-Tat11 peptide membrane-perturbing action by whole-cell patch-clamp recording | |
| Autori: | ||
| Data di pubblicazione: | 2014 | |
| Rivista: | ||
| Parole Chiave: | Animals; CHO Cells; Cell Membrane Permeability; Cell-Penetrating Peptides; Cricetinae; Cricetulus; Membrane Potentials; Patch-Clamp Techniques; Recombinant Fusion Proteins | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.3390/molecules19079228 | |
| Handle: | http://hdl.handle.net/11384/60405 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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