Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.

Simultaneous intracellular chloride and pH measurements using a GFP-based sensor

MARCHETTI, LAURA;ALBERTAZZI, LORENZO;BELTRAM, Fabio
2010

Abstract

Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.
File in questo prodotto:
File Dimensione Formato  
Simultaneous_chloride_and_pH_measurement_in_vivo_using_a_combined_genetically_encoded_sensor_2010.pdf

Accesso chiuso

Tipologia: Altro materiale allegato
Licenza: Non pubblico
Dimensione 610.86 kB
Formato Adobe PDF
610.86 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11384/6272
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 152
  • ???jsp.display-item.citation.isi??? 145
social impact