The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.
Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies
CHIRICHELLA, MICHELE;LISI, SIMONETTA;FANTINI, MARCO;GORACCI, MARTINA;CALVELLO, MARIANTONIETTA;ARISI, Ivan;DI PRIMIO, CRISTINA;CATTANEO, ANTONINO
2017
Abstract
The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.File in questo prodotto:
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