Tyrosinase-catalyzed oxidation of 17b-estradiol: structure elucidation of the products formed beyond cathecol estrogen quinone

This paper reports a systematic characterization of the products formed by oxidation of 17 beta-estradiol (1) with tyrosinase/O-2 at low concentrations of physiological relevance. With the substrate at 1-10 nM concentration, the main reaction products included, beside the catechol estrogens 2-hydroxyestradiol (2) and 4-hydroxyestradiol (3), 6-oxo-2-hydroxyestradiol (4), 9,11-dehydro-2-hydroxyestradiol (6), 6,7-dehydro-2-hydroxyestradiol (7), and 9,11-dehydro-4-hydroxyestradiol (10). At higher estradiol concentrations, e.g., 1 mu M, 6,7,8,9-dehydro-2-hydroxyestradiol (5) and the dimeric products 8 and 9 were also formed. The origin of these products from oxidative routes of 2 and 3 was established. Overall, the results of this study disclose novel aspects of the reactivity of 1 with the tyrosinase/O-2 system and provide the first inventory of the oxidation products of catechol estrogen quinones.