A single-step method to coat and bifunctionalize water-reduced gold nanoparticles (NPs) with two distinct reactive groups is reported. The coating is based on a peptide that bonds to the NPs surface by its N-cysteine amino acid, terminates with a C-terminal lysine, and stabilizes the colloids, thanks to the surface organization provided by the rest of the non-polar chain. The process yields stable, noncytotoxic NPs presenting reactive amine and carboxylic groups on the surface; these allow rapid, selective and modular conjugation of virtually any chosen biomolecule or fluorophore. Functionalized and conjugated nanostructures are analyzed by electrophoresis, SEM, SERS; their biocompatibility and delivery capability are tested by cellular-uptake experiments.
|Titolo:||Single-step bifunctional coating for selectively conjugable nanoparticles|
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1039/c0nr00350f|
|Appare nelle tipologie:||1.1 Articolo in rivista|