Aim: Boron nitride nanotubes (BNNTs) are tubular nanoparticles with a structure analogous to that of carbon nanotubes, but with B and N atoms that completely replace the C atoms. Many favorable results indicate BNNTs as safe nanomaterials; however, important concerns have recently been raised about ultra-pure, long (˜10 µm) BNNTs tested on several cell types. Materials & methods: Here, we propose additional experiments with the same BNNTs, but shortened (˜1.5 µm) with a homogenization/sonication treatment that allows for their dispersion in gum Arabic aqueous solutions. Obtained BNNTs are tested on human endothelial and neuron-like cells with several independent biocompatibility assays. Moreover, for the first time, their strong sum-frequency generation signal is exploited to assess the cellular uptake. Results & conclusion: Our data demonstrate no toxic effects up to concentrations of 20 µg/ml, once more confirming biosafety of BNNTs, and again highlighting that nanoparticle aspect ratio plays a key role in the biocompatibility evaluation.

Cytocompatibility evaluation of gum Arabic-coated ultra-pure boron nitride nanotubes on human cells

DE VITO, GIUSEPPE;
2014

Abstract

Aim: Boron nitride nanotubes (BNNTs) are tubular nanoparticles with a structure analogous to that of carbon nanotubes, but with B and N atoms that completely replace the C atoms. Many favorable results indicate BNNTs as safe nanomaterials; however, important concerns have recently been raised about ultra-pure, long (˜10 µm) BNNTs tested on several cell types. Materials & methods: Here, we propose additional experiments with the same BNNTs, but shortened (˜1.5 µm) with a homogenization/sonication treatment that allows for their dispersion in gum Arabic aqueous solutions. Obtained BNNTs are tested on human endothelial and neuron-like cells with several independent biocompatibility assays. Moreover, for the first time, their strong sum-frequency generation signal is exploited to assess the cellular uptake. Results & conclusion: Our data demonstrate no toxic effects up to concentrations of 20 µg/ml, once more confirming biosafety of BNNTs, and again highlighting that nanoparticle aspect ratio plays a key role in the biocompatibility evaluation.
2014
Settore M-PSI/02 - Psicobiologia e Psicologia Fisiologica
biocompatibility; boron nitride nanotubes; gum Arabic; HUVECs; SH-SY5Y;
File in questo prodotto:
File Dimensione Formato  
Ciofani14_NMUK.pdf

Accesso chiuso

Tipologia: Published version
Licenza: Non pubblico
Dimensione 6.99 MB
Formato Adobe PDF
6.99 MB Adobe PDF   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11384/27390
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 62
  • ???jsp.display-item.citation.isi??? 54
social impact